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. 2016 Feb 3;48(4):1608–1616. doi: 10.3892/ijo.2016.3373

Figure 3.

Figure 3

Scrophularia orientalis extracts induce ER calcium store depletion. MYCN2 cells were loaded with fluo-4 and ER-tracker and time-lapse confocal experiments were performed. 35D extract was applied to the cells. The extracellular solution contained 1 mM of calcium. At 200 sec, cytoplasmic calcium levels were elevated and ER localization displays a decrease in calcium levels (close-up) (A). (B) The same experiment was performed. The external solution contained 10 mM EGTA decreasing extracellular-free calcium to ~10 nM. Cytoplasmic calcium levels were elevated after 400 sec and ER localized calcium was decreased.