Figure 5.

Scrophularia orientalis extracts induce apoptosis in neuroblastoma (NB) cells. MYCN2 cells were grown with and without induction of MYCN overexpression for 96 h and were treated the last 24 h of the experiment with 500 nM doxorubicin or 8 μg/ml of extract 35D. Whole cell lysates were analyzed by western blotting to determine protein level of cleavage of caspase-3 (A) and PARP (B) as well as PCNA. For quantification of caspase-3 protein levels were normalized on β-tubulin levels (C). For quantification of PARP cleavage fragments were normalized on β-tubulin and cleaved PARP fragment was normalized on full length PARP fragment (D).