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. 2016 Jan 14;23(3):263–271. doi: 10.1038/gt.2015.111

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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AAV serotype-dependent general CNS biodistribution. Following six site-directed vector administration (a), representative tissue sections of interest (b) were analyzed for percentage of area with GFP expression. Differences in combined percentage GFP area for all four sections were quantified for each serotype based on genotype (c), and rAAV-GFP expression was quantified for each selected brain section by serotype in 3-month-old control (d) and MPS IIIB littermates (e). Two-way ANOVA was used to assess differences within tissue section based on serotype, and one-way ANOVA was used to test individual differences between serotype, n=3–6/cohort *P<0.05; **P<0.01. Data represented as mean±s.e.m.