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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Cell Biochem. 2016 Feb 20;414(0):153–169. doi: 10.1007/s11010-016-2668-3

Figure 2.

Figure 2

OMC32 (0.5mg) polypeptide was coupled to an AminoLink Plus resin at pH 10.0. Bovine cauda sperm was extracted in TNI containing 0.1% Triton X-100 (TNI-TX) at 4°C for 1 hr and centrifuged at 100,000 X g for 30 min in a Beckman SW40 rotor. The supernatant obtained after centrifugation was applied to the OMC32 coupled column. The column was washed with 10 column volumes of TNI-TX solution and then eluted with 0.1M Glycine-HCl, pH 2.5. Lane 1 shows the pattern of polypeptides in the detergent soluble fraction of bovine cauda sperm. Lane 2 reveals the unbound polypeptides to the OMC32 coupled column. The acid eluted fraction of the OMC32 affinity column was stained with silver (lane 3). Two major bands, 1 & 4, and two minor bands, 2 & 3, as shown in Fig.2, lane 3, are subjected to MALDI-TOF-TOF analysis for the proteomic identification.