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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Cell Biochem. 2016 Feb 20;414(0):153–169. doi: 10.1007/s11010-016-2668-3

Figure 8.

Figure 8

Matched phase contrast (panel A’) and fluorescence (panel A) photomicrographs of LPC-induced acrosome reacted bovine sperm immunostained with anti-IZUMO1. Note that the cells show specific staining at the equatorial segment, demonstrating the re-localization of entire pool of IZUMO1 over the equatorial segment in acrosome-reacted spermatozoa. Matched phase contrast (B’ & C’) and fluorescence (B & C’) images of noncapacitated sperm (Fig. B & B’) and acrosome-reacted sperm (Fig. C & C’) stained with FITC-conjugated PSA. PSA stained confirmed the LPC-induced acrosome reaction of bovine sperm.