Fig. 3.

Slug, a known transcription factor for EMT-related proteins, is not differentially expressed in bone versus visceral metastases, but is seen in a subset of cells displaying EMT-like behavior. Expression of Cytoplasmic and nuclear Slug expression is not significantly different in bone metastases vs visceral metastases. Staining intensities (intense, moderate, weak, or no staining) are described in the inset in Fig. 1. Patients—represents the number of patients represented, sites—represents the number of metastases. To highlight the epithelial phenotype, images of serial sections of a bone metastasis stained for Slug and Pan-cytokeratin are shown to the right. White arrows highlight epithelial cells with elongated nuclei and the black arrows highlight cells with round nuclei. Bar = 40 microns