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. 2016 Jan 21;23(3):272–282. doi: 10.1038/gt.2015.112

Figure 1.

Figure 1

AAV serotypes show diverse transduction patterns in the motor cortex. (a) Schematic representation of an AAV cis-shuttle plasmid expressing eGFP under CMV promoter and trans-plasmid encoding for AAV2 rep proteins and capsids. (b) Experimental approach showing direct injection of different AAV serotypes and simultaneous injection of red fluorescent microspheres into the CST that lies within the dorsal funiculus of the spinal cord. All injections were performed at P30, and the tissue was collected at P60. (c) Schematic representation of the coronal section of the brain. The red dots represent location of CSMN, and the green arrow depicts the location of AAV injection. Boxed areas, which represent the motor cortex (f), striatum (g) and the corpus callosum (h), are imaged below for each serotype tested. (d) Representative section showing AAV2-2 injection site in the motor cortex. (e) Representative images of AAV2-2-transduced neurons that are also retrogradely labeled with red fluorescent microspheres, confirming that the site of injection has been the motor cortex. (f) Representative images of cells transduced with AAV2-1, AAV2-2, AAV2-5, AAV2-6, AAV2-7, AAV2-8 and AAV2-9 in the motor cortex. (g, h) Representative images of eGFP+ axon bundles located at the striatum (g) and at the corpus callosum (h). (i) Representative images of dorsal funiculus with eGFP+ axons in the CST of the cervical spinal cord. Scale bars: 100 μm (d), 10 μm (e), 50 μm (g–i).