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. 2016 Jan 21;23(3):272–282. doi: 10.1038/gt.2015.112

Figure 4.

Figure 4

AAV2-2 with CBA promoter transduces both WT and diseased CSMN. (a) Experimental approach showing direct injection of AAV2-2 CBA. Injections were performed at P30 and P60, and the tissue was collected at P60 and P90, respectively. (b) Bar graphs represent mean percentage ±s.e.m. of CSMN (eGFP+ cells, expressing Ctip2) and CPN (eGFP+ cells, expressing SATB2) transduction in both WT and hSOD1G93A mice at P60 and P90. (c) Representative images of P60 WT neurons transduced with AAV2-2 encoding eGFP under the CBA promoter. Arrows indicate eGFP+ neurons expressing Ctip2 and not SATB2. (d) Representative images of hSOD1G93A neurons transduced with AAV2-2 encoding eGFP under the CBA promoter. Arrows indicate eGFP+ neurons expressing Ctip2 and not SATB2. No statistical differences were detected by one-way ANOVA and Tukey's multiple comparison test. Scale bar=20 μm.