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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Cancer Prev Res (Phila). 2016 Jan 22;9(3):215–224. doi: 10.1158/1940-6207.CAPR-15-0419

Figure 5. Topical treatment with rapamycin prior to SSL causes hyperphosphorylation of Akt (S473) in mouse epidermis which is inhibited by PHT-427.

Figure 5

Female SKH-1 hairless mice (n = 3) were pretreated three times (48hr, 24hr, and 1hr) with rapamycin (50nmol/back) prior to acute solar simulated light (SSL) treatment at the dose used in Figure 1. Mice were then sacrificed along a timecourse up to 4hr and epidermal proteins were extracted for Western blot analysis (0hr indicates sacrifice immediately after UV exposure, A). To confirm the delayed hyperphosphorylation of Akt (S473) after SSL in the presence of rapamycin, new mice were treated as described above and harvested at 6hr and 24hr post SSL for Western blot analysis of epidermal lysates (B, C). A third acute mouse experiment was performed to test the effects of PHT-427 on p-Akt (S473) in SSL-exposed epidermis. Mice were treated with rapamycin or PHT-427 (3.7umol/back) or both as described above and harvested 6hr post SSL exposure. Epidermal lysates were blotted for p-Akt (S473), p-S6 Ribosomal Protein or beta-tubulin as a loading control.