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Induced mutagenesis in Escherichia coli MG1655 in presence or absence of carvacrol, citral, (+)-limonene oxide, and ampicillin. Bacteria were grown in tryptic soy broth with yeast extract added, supplemented with 150 μL/L carvacrol (Car), 750 μL/L citral (Cit), 562.5 μL/L (+)-limonene oxide (Lim) and 1 mg/L ampicillin (Amp) or no compound added (control). The frequency of the rifampicin-resistant (RifR) mutants was measured. Data are means ± standard deviations (error bars).
