Figure 2.

Inactivation of Escherichia coli MG1655 wild type strain (WT) and its derivative strains, obtained by selection with carvacrol (CAR), citral (CIT) and (+)-limonene oxide (LIM), after lethal treatments with: 200 μL/L of carvacrol during 15 min (A); 300 μL/L of citral during 20 min (B); 500 μL/L of (+)-limonene oxide during 60 min (C); heat treatment at 55°C during 26 min (D); and a PEF treatment at 35 kV/cm for 50 pulses (E). Treatment medium was citrate-phosphate buffer of pH 7.0. Data are means ± standard deviations (error bars).