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. 2015 Dec 18;30(3):594–604. doi: 10.1038/leu.2015.285

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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EBV-miR-BHRF1-2 downregulates PRDM1 protein levels in LCL cells. (a) CCL159 or CCL156 cells were transfected either with 1 μM of EBV-miR-BHRF1-2 inhibitor, or with 1 μM mirvana miRNA inhibtior negative control. PRDM1 mRNA level was measured 48 h post transfection, and normalized to negative control. (b) Whole cell lysates were prepared from the above indicated transfectants at 48 h. PRDM1 protein expression was determined by western blotting. Lamin B was included as a loading control. Note: The doublet appears to be artifactual and was not seen in the immunoblots in Figures 3c and 6b. (c) Normalized PRDM1 protein was expressed as a percentage relative to the negative control, and graph was plotted using the prism 6 software (La Jolla, CA, USA). Error bars represent the s.e.m. from technical replicates. *P<0.05.