Table 3.

PCR cyclic condition of virulence genes.

Gene	Initial denaturation	35 cycles			Final extension
		Denaturation	Annealing	Extension
Flagellin gene	94°C	94°C	52°C	72°C	72°C
	5 min	45 s	45 s	1 min	10 min
Campylobacter adherence gene	94°C	94°C	45°C	72°C	72°C
	5 min	1 min	45 s	1 min	8 min
Invasion associated marker	94°C	94°C	52°C	72°C	72°C
	5 min	1 min	1 min	1 min	5 min
Flagellar synthesis and modification, flgR	95°C	95°C	54°C	72°C	72°C
	5 min	1 min	1 min	1 min	5 min
Cytolethal distending toxin subunit B gene	95°C	95°C	57°C	72°C	72°C
	5 min	30 s	30 s	30 s	10 min

PCR=Polymerase chain reaction