TABLE 3.
SAXS data collection and derived parameters for CTP1L
Rg, radius of gyration; Dmax, maximal particle dimension; Vp, Porod volume; Vex, particle excluded volume.
| Parameters | Values |
|---|---|
| Data collection | |
| Instrument | EMBL X33 beam line (DORIS-III, DESY, Hamburg) |
| Beam geometry (mm2) | 2.0 × 0.6 |
| Wavelength (Å) | 1.54 |
| s range (Å−1)a | 0.01–0.6 |
| Exposure time (s) | 8 × 15 |
| Concentration range (mg/ml) | 0.2–4.0 |
| Temperature (K) | 293 |
| Structureb | |
| I(0) (relative) (from p(r)) | 88.6 ± 2 |
| Rg (Å) (from p(r)) | 40 ± 1 |
| I(0) (cm−1) (from Guinier) | 85.3 ± 0.3 |
| Rg (Å) (from Guinier) | 37 ± 1 |
| Dmax (Å) | 138 |
| Porod volume estimate (Å3) | 94,520 ± 10,000 |
| Excluded volume estimate (Å3) | 128,000 ± 10,000 |
| Dry volume calculated from sequence (monomeric/dimeric) (Å3) | 40,727/81,453 |
| Molecular mass determination | |
| I(0) (cm−1) BSA (66,000 Da) | |
| Mr (from I(0)) | 65,160 ± 5000 |
| Mr (from Porod volume (Vp/1.6)) | 59,075 ± 5000 |
| Mr (from excluded volume (Vex/2)) | 64,000 ± 5000 |
| Calculated monomeric Mr from sequence | ∼32,840 |
a Momentum transfer s = 4πsin(θ)/λ.
b Values reported for merged data sets (CTP1L: 1.0 and 4.0 mg·ml−1).