FIGURE 2.

Treatment of HAECs with cytokine IL-17 increases the non-treated monocyte adhesion to HAECs. A, IL-17-treated HAECs have increased THP-1 cell adhesion. HAECs were treated with IL-17 (100 ng/ml) for the indicated time, and untreated labeled monocytes were allowed to adhere onto the endothelial monolayer. IL-17 treatment increased THP-1 monocytic cell adhesion onto HAECs in a time-dependent manner. The adhesion is shown as a percentage of the control group. B, untreated primary human monocyte adhesion to HAECs treated with IL-17 gradually increased up to 24 h. C, fluorescence microscopic images of labeled primary human monocyte adhered to HAECs. Data are presented as means ± S.E. (error bars) (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus control.