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. 2016 Jan 13;291(10):4955–4965. doi: 10.1074/jbc.M115.693499

FIGURE 3.

FIGURE 3.

DHA treatment enhances Akt and S6K activities and up-regulates Tau and CRMP2 expression. A, Western blotting analysis of cell lysates prepared from neurons treated with or without DHA for 3 DIV. B, quantified values of expression levels of Tau and CRMP2. The values were normalized to GAPDH expression (Tau, n = 5; CRMP2, n = 6). C, quantified values of phosphorylation levels of Ser(P)-473 Akt, Thr(P)-308 Akt, Thr(P)-389 S6K, and Thr(P)-421/Ser-424 S6K. The values were normalized to each expression level of the total protein, respectively (n > 3). D, mRNA expression quantified by real-time quantitative PCR. The values were normalized to GAPDH expression (n = 5). E, Western blotting analysis of cell lysates prepared from neurons treated with 1 μm DHA, stearic acid, oleic acid, or arachidonic acid for 3 DIV. Error bars indicate mean ± S.E., Mann-Whitney U test. *, p < 0.05; **, p < 0.01; n.s., not significant.