FIGURE 6.

CDN1163 improves mitochondrial efficiency in the liver of ob/ob mice. All determinations were performed on harvested liver tissues at the end of the study (i.e. day 50). qRT-PCR analyses of genes involved in mitochondrial biosynthesis (A) and mitochondrial DNA contents (B). C, Western blot analyses and quantification of proteins involved in OXPHOS and qRT-PCR analyses of OXPHOS genes (D), ATP content (E), and antioxidant enzymes (G) in vehicle (Ob)- versus CDN1163-treated obese (Ob+CDN) mice. F, AMPK phosphorylation (pAMPK) and densitometry (normalized to total AMPK (tAMPK)) in liver samples from lean, vehicle-treated (Ob) versus CDN1163-treated obese (Ob-CDN) mice. GAPDH is a loading control. Data are expressed as the means ± S.E. from at least three experiments; #, p < 0.05 and ##, p < 0.01 obese versus CDN-treated obese mice; **, p < 0.01 versus lean and ***, p < 0.001 CDN1163 versus vehicle.