FIGURE 5.

NatA does not acetylate peptides or proteins corresponding to reported lysine substrates. Recombinant human Naa10/Naa15 (NatA) was used for these experiments. A, radioactive filter binding assay of peptide acetylation in the presence or absence of Naa10. An N-terminal SASE peptide was used as a positive control as reported previously (9). B, radioactive TCA precipitation assay of protein acetylation with Runx2 and MSRA in the presence of absence of Naa10. The experiments were carried out in triplicate, and error bars are indicated.