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. 2016 Jan 11;291(10):5270–5277. doi: 10.1074/jbc.M115.709428

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Naa10 and NatA do not interact with MSRA or Runx2. A, GST pulldown assays of Naa10 using GST tagged Runx2 and MSRA. Coomassie staining of SDS-PAGE is shown for both the input (left) and elution (right). Corresponding migrating proteins in the gels are labeled. B, GST pulldown assays of Naa10/Naa15 (NatA) using GST-tagged Runx2 and MSRA. The experiment was carried out as described in A. C, as a positive control, pulldowns of NatA were carried out using GST-tagged Naa50. Asterisks indicate free hydrolyzed GST.