FIGURE 12.

Modulation of spreading and contractility by ROCK and MLCK signaling pathways in SH-SY5Y cells. A, phase-contrast images of SH-SY5Y cells after 24 h of culture on collagen and kassinin substrates after treatment with 1 μm ML7 (MLCK inhibitor) or 1 μm Y27632 (ROCK inhibitor) for 1-h duration. Scale bar, 20 μm. B, quantification of cell spreading area in drug-treated cells compared with controls. Error bars, S.E. Statistical significance, **, p < 0.005. C, de-adhesion times of control and drug-treated cells cultured on collagen and kassinin. Error bars, S.E. (**, p < 0.005). Whereas both ML7 and Y-27632 treatment led to delayed de-adhesion on collagen substrates, cells were sensitive to ML7 treatment only on kassinin substrates.