FIGURE 16.

Cell adhesion on PAAs and its corresponding amyloid fibrils. A, phase-contrast images of SH-SY5Y cells after 24 h of culture on freshly dissolved PAAs and their corresponding amyloid fibrils. Scale bars, 100 μm. B, quantification of cells adhered on PAA and their fibrils. Error bars, S.E. C, morphology of cells adhered on PAAs and their fibrils is quantified by calculating the spreading area of the cells. Increased cell number and spreading area on PAA fibrils suggest stronger cell adhesion on PAA amyloid fibril-coated surface. D, cytoskeletal organization and focal adhesion formation in SH-SY5Y cells on PLL and its corresponding amyloid fibril. Phalloidin (green), FAK (red), and DAPI (blue) were used to visualize F-actin, focal adhesions, and nuclei, respectively. Scale bar, 20 μm. E, β1 integrin expression profile in SH-SY5Y cells cultured on PLL and PLL fibril-coated glass coverslips. The higher integrin expression and large FAK clusters were observed in cells adhered on PLL fibrils, indicating stronger cell adhesion on amyloid fibrils than on their soluble counterparts.