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. 2016 Jan 5;291(10):5309–5319. doi: 10.1074/jbc.M115.693218

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — BER activities of 135-bp duplexes with centrally positioned Sp lesions in cell-free extracts from wild (NEIL1^+/+) and KO (NEIL1^−/−) MEFs. The 135-mer modified strands contained the centrally positioned sequences: 5-CCATC(Sp-S)CTACC (#1), 5′-CCACCAAC(Sp-S)CTACCACC (#2), or 5′-CCACCAAC(Sp-R)TCACCACC (#3); the remaining base sequences in the 135-mer duplexes with #2 and #3 are shown in Fig. 1B. Lanes 1 and 11, oligonucleotide size markers. The BER activities (yield of 67-mer fragments) are strongly reduced in NEIL1^−/− sequences in all three sequence contexts.