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Expression and purification of lipid-free apoA-IΔ185–243. apoA-IΔ185–243 was expressed and purified from bacteria as described under “Experimental Procedures.” a, SDS-PAGE analysis of wild-type apoA-I (lane 1) and apoA-IΔ185–243 (lane 2). b, resolution and molecular weight determination of 14N and 15N apoA-IΔ185–243 using mass spectrometry.
