TABLE 1.
Cross-link | Peptides involveda | Mod.b | Sample | Massc |
|
---|---|---|---|---|---|
14N | 15N | ||||
Da | |||||
Lys88–Lys94 | 84QEMSKDLEEVKAK96 | XL | M | 1671.86 | 1688.81 |
XL | D | 1671.86 | 1688.81 | ||
Lys12–Lys23 | 11VKDLATVYVDVLKDSGR27 | XL | M | 2015.13 | 2037.07 |
XL | D | 2015.12 | 2037.06 | ||
Lys23–Ser25 | 11VKDLATVYVDVLKDSGR27 | XL, H | M | 2171.22 | 2193.16 |
XL, H | D | 2171.21 | 2193.15 | ||
Lys133–Lys140 | 132QKLHELQEKLSPLGEEMR149 | XL | M | 2302.24 | 2329.16 |
XL | D | 2302.23 | 2329.15 | ||
Lys133–Ser142 | 132QKLHELQEKLSPLGEEMR149 | XL | M | 2302.24 | 2329.16 |
D | NDd | ND | |||
Lys140–Ser142 | 132QKLHELQEKLSPLGEEMR149 | XL, H | M | 2458.31 | 2485.24 |
D | ND | ND | |||
Lys106–Lys107 | 97VQPYLDDFQKKWQEEMELYR116 | XL | M | 2782.39 | 2811.32 |
D | ND | ND |
a Lysines or serines involved in cross-links are in bold type.
b Mod., chemical modifications; XL, one complete cross-link (+138.068 Da); H, one hydrolyzed cross-linker (+156.079 Da).
c Experimentally derived monoisotopic mass for each peptide with each isotope and the combinations.
d ND, not detected. These ions were detectable in one sample (i.e. monomer or dimer) but not in the other.