Figure 5.
Gadd45a exerts reactive oxygen species scavenging effects by upregulating the expression of antioxidant enzymes. A: The effects of Gadd45a on ROS generation in CCl4-pretreated HSCs treated with pcDNA3.1, PCDNA3.1-Gadd45a, scrambled siRNA, and Gadd45a siRNA were detected by flow cytometry; B: Effects of Gadd45a on HO-1 and NQO1 RNA expression in CCl4-pretreated HSCs treated with pcDNA3.1, PCDNA3.1-Gadd45a, scrambled siRNA, and Gadd45a siRNA; C: Effects of Gadd45a on HO-1 and NQO1 protein expression in CCl4-pretreated HSCs treated with pcDNA3.1, PCDNA3.1-Gadd45a, scrambled siRNA, and Gadd45a siRNA. HSC: Hepatic stellate cells; ROS: Reactive oxygen species.