Effects on total neurons-PGP9.5 protein expression in immunohistochemical staining. Tissue with brown granular deposits were positive reaction (as arrows indicated; -: 50 μm, magnification × 400). A, B: In jejunum; D, E: In ileum; G, H: In proximal colon; C, F, I: Comparison of the mean density of PGP9.5-positive expression between two groups. aP < 0.05 (n = 5 for each group).