Figure 10. Levels of proinflammatory protein markers in hearts from young or old WT and MIF^−/− mice treated with or without the autophagy inducer rapamycin.

Rapamycin treatment (2 mg/kg body weight/d, i.p.) were initiated when MIF^−/− mice were 22-month old for 2-month. (A) Representative gel blots depicting levels of IκB, p-IκB and IL-6 using specific antibodies (α-tubulin and GAPDH were used as the loading control); (B) p-IκB expression; (C) IκB expression; (D) p-IκB-to-IκB ratio; and (D) IL-6 expression; Mean ± SEM, n = 3–6 mice per group, *p < 0.05 vs. WT young group, ^#p < 0.05 vs. WT old group, ^†p < 0.05 vs. MIF^−/− old group.