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. 2016 Mar 4;6:22488. doi: 10.1038/srep22488

Figure 6. Effect of MIF silencing and replenishment on doxorubicin-induced cell senescence in cultured H9C2 myoblast cells.

Figure 6

(A) Representative micrographs depicting levels of S-A β-gal measured using C12FDG (33 μM) in H9C2 cells. H9C2 cells treated with MIF siRNA or scrambled siRNA for 48 hrs were incubated with doxorubicin (DOX, 0.1 μM) for 24 hrs, followed by standard (DMEM without FBS) culture media for another 24 hrs. Recombinant mouse MIF (rmMIF, 10 ng/ml) was added into H9C2 cells treated with scrambled or siRNA MIF; and (B) Quantitative analysis of S-A β-gal levels in H9C2 cells. Mean ± SEM, n = 50 cells per group, *p < 0.05 vs. control group, #p < 0.05 vs. DOX-scramble siRNA group, p < 0.05 vs. DOX MIF siRNA group.

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