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. 2016 Mar 2;7:10716. doi: 10.1038/ncomms10716

Figure 2. Functional analysis of the HUH nuclease and SF1B helicase domains.

Figure 2

(a) Transposition by wild-type (WT) Helraiser transposase and its mutants in HeLa cells. Data are represented as mean±s.e.m., n=3 biological replicates. (b) Cleavage of single-stranded DNA oligonucleotides by the Helraiser transposase in vitro. (c) DNA-binding assay with the Helraiser transposase and its point mutant and truncated derivatives. 0.3–5 × values represent molar ratios of protein to DNA. (d) Colorimetric ATPase assay with the WT and K1068Q mutant transposase protein. PPi, pyrophosphate release. Data are represented as mean ± s.d., n=3 biological replicates.