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. 2016 Mar 2;7:10716. doi: 10.1038/ncomms10716

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(a) Helitron circle donor plasmid (pHelRCD) and Helraiser transposase-generated Helitron circle (pHelRC). White arrow, Kan/SV40 promoter; white circle, polyA signal. (b) Transposition of Helitron circles. Data are represented as mean±s.e.m., n=3 biological replicates. (c) PCR detection of Helitron circles generated with HelR, HelRMutHP and HelRΔHP transposons. HelRMutHP, transposon with a deletion of the last 9 nt of the palindrome; H₂O, no template control. (d) Relative transposition efficiencies of pHelRCpuro and pHelRCΔHPpuro. Data are represented as mean±s.e.m., n=4 biological replicates.