Figure 5. Activation of the p53/p21 pathway resulting from oxidative stress induced by 150 μM H₂O₂.

(A) The addition of H₂O₂ increased p53 and p21 expression at the mRNA level, but did not increase p38 or p16 expression at the mRNA level. (B) Ac-p53, T-p53 and p21 protein expression were detected by Western blotting with H₂O₂ or not. (C) The addition of H₂O₂ increased the acetylated p53 (lys382) and p21 protein expression level. (D) phos-p38, T-p38 and p16 protein expression were detected by Western blotting with H₂O₂ or not. (E) The addition of H₂O₂ did not increase phosphorylated p38 or p16 protein expression level. The gels have been run under the same experimental conditions. Three independent experiments were performed on the degenerative human CEP cell from three patients, and the data was denoted in terms of mean ± SD. *P < 0.05. NS: P > 0.05.