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. 2016 Mar 4;6:22582. doi: 10.1038/srep22582

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Copyright © 2016, Macmillan Publishers Limited

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Quantitative PCR was used to measure the mRNA abundance of key genes involved in HPI axis function in 48 hpf embryos. Measured genes were: mc2r (a,b), star (c,d), and 11β-hydroxylase (e,f) from embryos injected with either cortisol antibody (Cort AB) or yeast-specific antibody (Yeast AB) (a,c,e) or with 32 pg of cortisol (Cort) or vehicle only control (Con) (b,d,f). Expression of 11β-hydroxylase and star were upregulated as a result of cortisol injection (relative to control embryos), and downregulated after cortisol antibody injection (relative to yeast antibody injected embryos), while expression of mc2r was upregulated only after cortisol injection. All data presented is mean ± SEM; *indicates significant differences (n = 6; each pool of 15 embryos; students t-test, p < 0.05).