Table 1.

Overview of four allergy test methods.

	Microtest	ISAC	ImmunoCAP 250	SPT
Method	Multiplex	Multiplex	Singleplex	Singleplex
	in‐vitro	in‐vitro	in‐vitro	in‐vivo
	Automated	Manual	Automated	Manual
Where	In clinic or laboratory	Laboratory	Laboratory	Clinic
Time to result	∼4 h	∼4 h	∼2 h	∼25 min
Reported results/assay	26	112	1	1
Components measured/assay	16	112	1	Not available
Extracts measured/assay	19	0	1	1
Sample volume	100 µl/26 allergens	30 µl/112 allergens	50 µl/allergen	0

SPT = skin prick test; ISAC = Immuno‐Solid phase Allergen Chip.