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. 2016 Feb 4;184(1):101–109. doi: 10.1111/cei.12752

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© 2016 British Society for Immunology

PMC Copyright notice

Anti‐HIV‐1 antibody‐dependent activation of killer immunoglobulin‐like receptor (KIR)2DL1⁺ and KIR2DL1⁻ natural killer (NK) cells from human leucocyte antigen (HLA)‐C2 carrying donors by autologous target cells. NK cells within whole blood from five HLA‐C2 carrying donors were stimulated using the autologous whole blood anti‐HIV‐1 antibody‐dependent activation assay. Data were collected by flow cytometry and CD3⁻CD56⁺KIR2DL1⁺ and CD3⁻CD56⁺KIR2DL1^– NK cells were assessed for interferon (IFN)‐γ production. The graph depicts the relative production of IFN‐γ by KIR2DL1⁺ and KIR2DL1⁻ NK cells from each donor.