Figure 1.

Arthritis index (AI) evaluation. An AI that expressed a cumulative score for all paws (maximum possible value = 16) was calculated for each animal. Animals were evaluated every 3 days after the infusion of the antibody cocktail for arthritis incidence and each paw was evaluated and scored individually on a scale of 0–4, with 4 indicating the most severe inflammation. Less severe arthritis was observed in mice in which high‐mobility group box 1 (HMGB1) was blocked by BoxA administration. The arthritis induced in HMGB1‐treated mice was similar to that observed in control collagen antibody induced arthritis (CAIA) mice. Arthritis induction was reduced in mice treated with the vascular endothelial growth factor (VEGF) inhibitor sFlt1; n = 5 mice per group. *P < 0.05 versus control CAIA mice.