Figure 1.

The design of the selection experiment, illustrating the global and local competition treatments. This scale of competition treatment was crossed with a multiplicity of infection (MOI) treatment (two levels), in which we imposed low relatedness by using 5000 infective juveniles (IJs) per dish or higher relatedness by using 250 IJs per dish. We used four independent selected lines in each treatment. Note that harvest (White) traps for individual cadavers in the local competition treatment were briefly screened for effective IJ production before being included in inocula pools for the next round of infection.