Table 5. Verification of Affymetrix data by RT PCR.
| Gene symbol | Gene Name |
RT PCR |
Affymetrix |
||
|---|---|---|---|---|---|
| FC | P-value | FC | P-value | ||
| NFKBIA | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha | 12.9 | 4.0E-06 | 6.9 | 2.0E-03 |
| RPE65 | Retinal pigment epithelium-specific protein 65kDa | 4.3 | 1.8E-07 | 2.8 | 2.7E-05 |
| CSF1 | Colony stimulating factor 1 (macrophage) | 6.8 | 1.8E-04 | 2.8 | 1.0E-02 |
| NFKBIZ | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta | 5.1 | 2.0E-03 | 2.6 | 2.0E-02 |
| RGR | Retinal G protein coupled receptor | 3.6 | 5.4E-05 | 2.3 | 2.0E-03 |
The table shows average fold change (increase) in mRNA levels upon incubation of human retinal pigment epithelial cells with sericin for 12 days as compared to controls. Data are average from triplicates with MAPRE1 and POLR5H as endogenous controls. RT PCR = real time polymerace chain reaction; FC = fold change.