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(a) Sirtuin-mediated deacylation reaction transfers fluorescently labeled acyl residues from lysine side chain to ADP-ribose. (b) Sirtuin-mediated deacylation reaction transfers quencher-containing acyl residue from lysine side chain to ADP-ribose. In both cases sirtuin activity causes an increase in the fluorescence signal. (Fl – fluorophore, Qu – quencher, ADPr – ADP ribose)
