Table 1. Kinetic constants for 3, 4, 4a and 5 and Sirt2.

Substrate	KM [μM]	10−3xkcat [s−1]	kcat/KM [M−1s−1]	Distance (No. of bonds)
4	17.7 ± 1.5	4.0 ± 0.1	224a	6
4a	1.2 ± 0.1	45.4 ± 1.8	38 600b	8
3	0.1 ± 0.02	23.8 ± 0.8	176 000a	11
5	15.3 ± 2.7	2.4 ± 0.1	156b	13

^ameasured using fluorescence spectrophotometer.

^bmeasured using MTP fluorescence reader (see supporting information for details). Data are presented as mean ± s.d. (n = 2).