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. 2016 Mar 4;6:22649. doi: 10.1038/srep22649

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Copyright © 2016, Macmillan Publishers Limited

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GST-CCR10 fusion protein containing the C-terminal tail (CT) of human CCR10 and GST (as a negative control) were immobilized on Glutathione sepharose beads and then incubated with (a) malignant melanoma cell line UKRV-Mel-4 lysates or (b) purified recombinant human anxA2, recombinant HIS-tagged human S100A10, or native anxA2-S100A10 tetramer isolated from porcine intestinal mucosa. Interacting proteins were analysed by immunoblotting with the indicated antibodies. Full uncropped scans of blots are shown in Supplementary Fig. S5.