Figure 4. Mapping of the S100A10 binding site in the CCR10 C-terminal tail.

(a) Linear representation of the C-terminus of CCR10 fused to GST, and the two truncation constructs T1 and T2. (b) Binding of GST-CCR10 CT, T1 and T2 to HIS-tagged S100A10 was analysed by pull-down and immunoblotting of the glutathione sepharose-bound proteins with the antibodies indicated. Blots are representative of at least five experiments. Full uncropped scans of blots are shown in Supplementary Fig. S5.