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. 2015 Dec 8;127(9):1085–1096. doi: 10.1182/blood-2015-05-644260

Figure 4.

Figure 4

Fibrin-internalizing cells display a low proliferation rate and express components of the plasminogen activation system. (A-E) Analysis of proliferation of fibrin-uptaking and fibrin-nonuptaking cells from fibrin implantation zones. (A-B) Representative examples of flow cytometry analysis of CD45-positive, fibrin-internalizing cells (A) and CD45-positive, fibrin-noninternalizing cells (B) stained with Ki-67 antibodies. (C) Flow cytometry of CD45-positive spleen cells (positive control). (D) Flow cytometry analysis of CD45-positive, fibrin-internalizing cells with primary antibody omitted (negative control). Diagrams show intensity of Ki-67 staining vs forward scatter. Boxes show gate for positive signal, and percent of cells in gate is indicated. (E) Enumeration of Ki-67–positive, CD45-positive fibrin-uptaking (left bar, N = 6) and CD45-positive, fibrin-negative (middle bar, N = 6) cells. Ki-67 staining of CD45-positive spleen cells (N = 4) are shown in the right bar. Data are shown as mean with standard deviation. P value was determined by Student t test (2 tailed). (F) Real-time PCR analysis of Plau (left bars) and Plaur (right bars) mRNA expression in fluorescence-activated cell sorting–isolated CD45-positive, fibrin-negative (open bars) and CD45-positive, CCR2-positive, fibrin-positive (cross-hatched bars). Data are shown as mean with standard deviation and were generated from 3 samples, each sample being a pool of cells isolated from 3 mice. Ab, antibody; FSC-A, forward scatter-A.