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(A) Newtonian resistance (Rn), which is representative of central airways resistance was assessed via SCIREQ® Flexivent small-animal ventilator. Nebulised doses of methacholine (MCh) in injectable saline were delivered via tracheal cannula. (B) Maximal Rn response to MCh is expressed as % saline control response (n = 5−12). *p < 0.05 compared to vehicle and to IAV treated mice.
