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. 2016 Mar 4;14:10. doi: 10.1186/s12915-016-0233-x

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© Koziol et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Expression of SFRP, posterior Wnt, and Frizzled receptor genes during late E. multilocularis protoscolex development. Whole-mount in situ hybridization signal is shown in green and nuclear DAPI staining in blue. PS stages 3 to 4: Stages 3 to 4 of protoscolex development following the system of Leducq and Gabrion [25]. Activated PS: Activated protoscoleces after isolation from metacestode vesicles and successive treatments with pepsin and sodium taurocholate. s, Suckers; r, Rostellum. Bars: 20 μm