Fig. 6.

Effect of TLR4 Knockout on the antitumor response of synthetic HPV-16 E7aa 43-62 long peptide vaccine. 3 × 10⁴ TC-1-Luc cells were submucosally injected into the right buccal area of C57BL/6 or C57BL/10ScNJ (TLR4−/−) mice (five per group). Three days after tumor injection, mice were vaccinated intratumorally with or without 50 μg of synthetic HPV-16 E7aa 43-62 peptide for four times in a 4-day intervals. Figure represents the Kaplan–Meier survival analysis of mice in TLR4 knockout experiment