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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Biochem Pharmacol. 2016 Feb 18;104:131–138. doi: 10.1016/j.bcp.2016.02.009

Figure 2. The activity and expression of WT hPXR and hPXR mutants in HepG2 cells.

Figure 2

(A) HepG2 cells transiently transfected with empty vector (EV), WT hPXR (hPXR) or hPXR mutant as indicated, CYP3A4-luc, and CMV-Renilla were treated with DMSO or different concentrations of agonists as indicated for 24 h prior to luciferase assay. (B) Western blot showing Flag-PXR (WT or mutants) protein levels in HepG2 cells upon (a) RIF [5 μM] and (b) DMSO treatment. The numbers below the protein bands indicate the relative intensity of the protein bands, with the wild-type Flag-PXR sample set as “1”. Anti-Flag antibody was used to detect Flag-PXR. Anti-β-actin was used to detect β-actin (as loading control).