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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Biochem Pharmacol. 2016 Feb 18;104:131–138. doi: 10.1016/j.bcp.2016.02.009

Figure 5. Mammalian two-hybrid assay to determine the interaction between WT or mutated hPXR and SRC-1.

Figure 5

Mammalian two-hybrid assays were performed in HepG2 cells transiently co-transfected with plasmids encoding Gal4-SRC-1 and the reporter gene pG5-luc, together with either empty vector pACT, or pACT fused to WT (pACT-hPXR) or mutated hPXR (W299V, W299G, W 299D) as indicated. The cells were treated with DMSO; RIF; or SR compound at the indicated concentrations. Luciferase assays were performed 24 h after the compound treatment. The relative luminescence for pG5-luc was determined by normalizing firefly luciferase activity to Renilla luciferase activity (Luciferase/Renilla).