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Molecular mass measurement of an AChBP subunit after labeling with AzEPI. The 25352 cluster represents the unmodified protein (AChBP) within which the mass increases of 16 and 33 Da may indicate the presence of methionine mono- and bissulfoxides. The second cluster corresponds to the singly EPI-nitrene-modified protein (EPI-N-AChBP) and a monooxidized derivative. The third very weak cluster indicates trace double labeling.
