Figure 2:

Traffic noise reduced sperm quantity and quality in White's treefrogs. (A) Frogs in the traffic noise group had significantly lower sperm counts than control frogs (P < 0.01, shown are means ± 95% confidence intervals). Analyses were conducted on logarithmically transformed data, which were then back transformed for presentation. (B) Frogs in the traffic noise group also had lower sperm viability (P < 0.01) at the end of the experiment relative to control frogs (n = 19). CFSE labels all cells, whereas viability dye labels only dead cells. More intense labelling with viability dye indicates an increase in dead cells. The plot shown is representative of all replicates. The mean proportions ± SD of live cells (upper left quadrant) and dead cells (upper right quadrant) are labelled for each group.