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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Thromb Haemost. 2015 Oct 20;13(11):2063–2075. doi: 10.1111/jth.13149

Fig. 4. The cryptic epitope of anti-metalloprotease domain mAb 6A6 is exposed by conformational activation of ADAMTS13.

Fig. 4

Binding of rADAMTS13, MDTCS and T2-CUB2 to 3H9 and/or 6A6 (both anti-metalloprotease domain mAbs) was tested in ELISA. Binding was detected with the anti-V5-HRP mAb. (A) Binding of rADAMTS13, MDTCS and T2-CUB2 to 3H9 and 6A6. Binding was calculated relative to the binding of MDTCS. (B) Binding of ADAMTS13, pre-incubated with the respective anti- T8 and anti-CUB mAb or Fab fragment, to 6A6. Binding was calculated relative to the binding of rADAMTS13. Differences were statistically analysed using the Student t-test. Error bars represent the SD of at least three independent experiments.